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Image Search Results
Journal: Cancer research
Article Title: BCL-2 IS A THERAPEUTIC TARGET FOR HYPODIPLOID B-LINEAGE ACUTE LYMPHOBLASTIC LEUKEMIA
doi: 10.1158/0008-5472.CAN-18-0236
Figure Lengend Snippet: A) Leukemic cells collected from the spleens of mice at the end of the trial were analyzed for levels of the prosurvival proteins: Bcl-2, Bcl-xL, MCL-1, and the proapoptotic BIM (EL (extra long), L (long) and S (short) isoforms), BAD, PUMA and BAX. Left panel corresponds to the control B-ALL: Ph+ and Ph-Like ALL while middle and right panels are LH and NH, respectively. Each lane represents individually xenografted mice: vehicle treated (V) and ABT-199 treated (ABT). Numbers correspond to the numbers given to the mice engrafted with each leukemia (1–6). Lane numbers highlighted in purple indicate mice with high IC50 to ABT-199 at end of trial. Solid line boxes highlight the mice with predominance of BIM and the dotted line boxes highlight mice with absence of BIM and predominance of BAD and PUMA. B) NALM-16 and Beck-1732 were exposed to increasing concentrations of ABT-199 for 24h and levels of the indicated proteins measured by WB. C) Beck-1732 cells were exposed to increasing doses of ABT-199 for 24 and 48h and IC50 levels determined.
Article Snippet: ATM (CST, 2873S), Actin-HRP (CST, 5125), BAD (Abcam, ab32445), BAX (Bio-Rad, MCA2738), Bcl-2 (Abcam, ab32124), Bcl-xL (Abcam, 32370), Bcl-w (LSBio, LS-C382259),
Techniques: Control
Journal: Cancer research
Article Title: BCL-2 IS A THERAPEUTIC TARGET FOR HYPODIPLOID B-LINEAGE ACUTE LYMPHOBLASTIC LEUKEMIA
doi: 10.1158/0008-5472.CAN-18-0236
Figure Lengend Snippet: A) Different leukemia cell lines from different hematopoietic lineages were subjected to 100 nM of ABT-199 for 24h. Proliferation was measured using ATP-bioluminescence (Cell Titer Glo) and %Max proliferation is shown. B) Bcl-2, Bcl-xL and BAX protein levels from different leukemia cell lines are shown. Vertical line has been inserted to indicate a repositioned gel lane. C) Levels of Bcl-2, Bcl-xL, MCL-1, BAX, BIM, BAD and PUMA proteins were measured in two near haploid cell lines (NALM-16, and MHH-CALL2). CCRF-CEM was used as negative control (low Bcl-2) and HL60 as positive control (high Bcl-2 levels) for sensitivity to ABT-199.
Article Snippet: ATM (CST, 2873S), Actin-HRP (CST, 5125), BAD (Abcam, ab32445), BAX (Bio-Rad, MCA2738), Bcl-2 (Abcam, ab32124), Bcl-xL (Abcam, 32370), Bcl-w (LSBio, LS-C382259),
Techniques: Negative Control, Positive Control